FIELD: biotechnology.
SUBSTANCE: present invention relates to biotechnology, in particular to a method of producing autologous dendritic cells, and can be used for cell immunotherapy of individuals with oncological diseases. To implement said method, monocytes are cultured in the presence of cytokines and lysate of autologous tumour cells for at least 7 days. At the same time on the membrane of activated dendritic cells in the incubation culture sodium glutamate is added to concentration of 5.34 mM - 10.25 mM and potassium pyruvate to concentration of 3.06 mM - 6.2 mM.
EFFECT: present invention enables to obtain autologous dendritic cells with high expression level of HLA-DR and co-stimulatory molecules CD80 and CD86 in oncological diseases.
1 cl, 2 tbl, 3 ex
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| DENDRITIC CELL GENERATION FROM MONOCYTIC DENDRITIC CELL PRE-CURSORS BY MEANS OF GM-CSF IN ABSENCE OF ADDITIONAL CYTOKINES | 2004 |
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| IMMUNOTHERAPY OF BREAST CANCER USING ANTIGEN-ACTIVATED DENDRITIC CELLS | 2016 |
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| METHODS RELATED TO ACTIVATED DENDRITIC CELLS COMPOSITIONS AND TO IMMUNOTHERAPEUTIC TREATMENT OF INDIVIDUALS WITH ADVANCED CANCER | 2016 |
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| COMBINED CELL TRANSPLANT BASED ON LYMPHOKINE-ACTIVATED KILLERS AND DENDRITE CELLS, METHOD FOR PRODUCTION THEREOF AND METHOD FOR TREATMENT AND PROPHYLAXIS OF MALIGNANT, INFECTIVE DISEASES AND IMMUNODEFICIENT CONDITIONS | 2006 |
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