RECOMBINANT PLASMID DNAS PX458D10A_MYC, PROVIDING MYC GENE KNOCKOUT IN HUMAN CELLS, METHOD OF PRODUCING HUMAN CELL LINE WITH MYC GENE KNOCKOUT AND HUMAN BREAST CANCER CELL LINE MDA-MB231_MYC WITH SUPPRESSED MYC GENE EXPRESSION Russian patent published in 2024 - IPC C12N15/07 C12N15/09 C12N15/85 

FIELD: biotechnology.

SUBSTANCE: invention relates to recombinant plasmid DNA PX458D10A_Myc. Plasmid provides the MYC gene knockout in the human breast cancer cell lines MDA-MB231_Myc, has a molecular weight of 5.7 MDa and is a plasmid vector PX458D10A, hydrolysed at a BpiI site, containing a replication initiation site of plasmid pBR322, fragment of 20 base pairs, complementary DNA of human MYC gene, having sequence SEQ ID NO 1-4. Present invention also relates to a method for producing a human breast cancer cell line MDA-MB231 with suppressed MYCgene expression, involving transfection of human cells with plasmid DNA PX458D10A_Myc.

EFFECT: present invention provides producing recombinant plasmids providing MYC gene knockout in MDA-MB231 human breast cancer cells, and the most acceptable and cost-effective method of producing a human cell line with reduced expression of the c-Myc transcription factor for research and development.

2 cl, 4 dwg, 2 ex