FIELD: cell biology.
SUBSTANCE: disclosed is a method of enriching CD4+- and CD8+-T-cells, which involves conducting in a closed CD4+-cell selection system and sampling CD8+-cells from a sample containing primary human T-cells, and combining said cells at a desired ratio of about 10:1 to 1:10. Also described is a method for producing genetically engineered T-cells, where T-cells are obtained using said method.
EFFECT: this group of inventions can be used in producing cells for adoptive therapy.
64 cl, 2 dwg, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHODS FOR CULTIVATION OF CELLS AND SETS AND DEVICE FOR THEM | 2016 |
|
RU2778411C2 |
| METHODS, SETS, MEANS AND DEVICES FOR TRANSDUCTION | 2016 |
|
RU2761555C2 |
| METHODS FOR CELL CULTIVATION AND SETS AND DEVICE FOR THEM | 2016 |
|
RU2761557C2 |
| OLIGOMERIC REAGENTS IN FORM OF PARTICLES AND THEIR APPLICATION METHODS | 2018 |
|
RU2777989C2 |
| METHODS FOR PRODUCTION OF COMPOSITIONS OF GENETICALLY ENGINEERED CELLS AND RELATED COMPOSITIONS | 2018 |
|
RU2790444C2 |
| PRODUCTION OF CONSTRUCTED CELLS FOR ADOPTIVE CELL THERAPY | 2017 |
|
RU2780156C2 |
| REAGENTS FOR REPRODUCTION OF CELLS EXPRESSING RECOMBINANT RECEPTORS | 2018 |
|
RU2783956C2 |
| METHODS AND COMPOSITIONS FOR OBTAINING GENETICALLY ENGINEERED CELLS | 2018 |
|
RU2795454C2 |
| COMPOSITIONS, PRODUCTS AND METHODS RELATED TO DOSING IN CELL THERAPY | 2018 |
|
RU2776823C2 |
| TRANSGENIC GENETIC LABELS AND METHODS OF USE | 2015 |
|
RU2822461C1 |
