FIELD: biotechnology.
SUBSTANCE: transformed CHO-K1 cell is proposed, which is not defective in endosomal acidification, producing aryl sulfatase B with high yield achieved due to its co-expression with recombinant sulfatase-modifying factor 1 as part of an expression vector under EF1a promoter. A vector expression structure for obtaining a transformed cell, which essentially contains sequences: CMVe/EF1a pro, BGH pA, a regulatory element that increases gene expression, and a gene encoding the expressed protein selected from ARSB and SUMF1 are also disclosed.
EFFECT: invention can be used for the production of active lysosomal aryl sulfatases for the treatment of diseases of lysosomal accumulation by enzyme replacement therapy.
7 cl, 8 dwg, 1 tbl, 5 ex
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