EXPRESSION PLASMID FOR HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE (FSH) IN CHO CELLS, EXPRESSION PLASMID FOR BETA-SUBUNIT OF HUMAN RECOMBINANT FSH IN CHO CELLS, CHO CELL PRODUCING HUMAN RECOMBINANT FSH, AND METHOD FOR PRODUCING ABOVE HORMONE Russian patent published in 2015 - IPC C12N15/63 C12N15/16 C12N15/79 C12N5/10 C12P21/02 

FIELD: medicine.

SUBSTANCE: invention refers to biotechnology and can be used for the recombinant production of human follicle-stimulating hormone (FSH). The procedure involves constructing an expression plasmid for human recombinant FSH in CHO cells; the above plasmid contains a region of origin of replication of the pUC plasmid, a beta-lactamase (bla) open reading frame and a bla gene prokaryotic promoter, a human Epstein-Barr virus terminal repeat (EBVTR) site, functional elements of Chinese hamster elongation factor 1 alpha gene, Kozak sequence; open reading frames of alpha- and beta-subunits of human FSH with stop-codons; an internal ribosomal entry site (IRES) of EMCV virus; an open reading frame of dihydrofolate reductase (DHFR) expressed as a part of tricystronic mRNA with the gene coding alpha- and beta-subunit of human FSH. The produced plasmid is used to transform a CHO cell to produce a FSH producer cell. The producer cell can be additionally transformed by the plasmid expressing the beta-subunit of human FSH.

EFFECT: invention enables substantially increasing the production effectiveness of human FSH in CHO cells.

15 cl, 7 dwg, 3 tbl, 10 ex