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2 697 273

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IPC

C07K14/59(2006-01-01)

C12N15/16(2006-01-01)

C12N15/85(2006-01-01)

C12N5/10(2006-01-01)

C12P21/02(2006-01-01)

(21) (22)

Application

2019105063, 2019-02-22

(24)

Start date

2019-02-22

(22)

Actual filing date

2019-02-22

(45)

Published

2019-08-13

(72)

Inventor

Monakhova Varvara SergeevnaKlimov Nikolaj AnatolevichKudling Tatyana ViktorovnaPigareva Natalya VasilevnaKarasev Maksim MikhajlovichShaldzhyan Aram ArutyunovichSimbirtsev Andrej Semenovich

(73)

Holder

Shatalin Dmitrij Aleksandrovich

METHOD OF PRODUCING HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE, PRODUCING CELL LINE AND PLASMID EXPRESSION VECTORS Russian patent published in 2019 - IPC C07K14/59 C12N15/16 C12N15/85 C12N5/10 C12P21/02

Abstract RU 2697273 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of therapeutic proteins, and can be used to produce human recombinant follicle-stimulating hormone (rhFSH). Plasmid expression vectors pTVK4p-FSHalpha and pTVK4n-FSHbeta are constructed, containing codon-optimized genes of alpha and beta subunit follicle-stimulating human hormone respectively. Said vectors are transformed CHO cells to produce cell line CHO-FSH 91 – producer rhFSH, which is used in production method rhFSH.

EFFECT: invention allows to increase production FSH human being for medical use.

4 cl, 9 dwg, 2 tbl, 7 ex

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RU 2 697 273 C1

Authors

Monakhova Varvara Sergeevna

Klimov Nikolaj Anatolevich

Kudling Tatyana Viktorovna

Pigareva Natalya Vasilevna

Karasev Maksim Mikhajlovich

Shaldzhyan Aram Arutyunovich

Simbirtsev Andrej Semenovich

Dates

2019-08-13—Published

2019-02-22—Filed