Show metadata Hide metadata

(19)

(11)

2 697 273

(13)

(51)

IPC

C07K14/59(2006-01-01)

C12N15/16(2006-01-01)

C12N15/85(2006-01-01)

C12N5/10(2006-01-01)

C12P21/02(2006-01-01)

(21) (22)

Application

2019105063, 2019-02-22

(24)

Start date

2019-02-22

(22)

Actual filing date

2019-02-22

(45)

Published

2019-08-13

(72)

Inventor

Monakhova Varvara SergeevnaKlimov Nikolaj AnatolevichKudling Tatyana ViktorovnaPigareva Natalya VasilevnaKarasev Maksim MikhajlovichShaldzhyan Aram ArutyunovichSimbirtsev Andrej Semenovich

(73)

Holder

Shatalin Dmitrij Aleksandrovich

METHOD OF PRODUCING HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE, PRODUCING CELL LINE AND PLASMID EXPRESSION VECTORS Russian patent published in 2019 - IPC C07K14/59 C12N15/16 C12N15/85 C12N5/10 C12P21/02

Abstract RU 2697273 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, specifically to recombinant production of therapeutic proteins, and can be used to produce human recombinant follicle-stimulating hormone (rhFSH). Plasmid expression vectors pTVK4p-FSHalpha and pTVK4n-FSHbeta are constructed, containing codon-optimized genes of alpha and beta subunit follicle-stimulating human hormone respectively. Said vectors are transformed CHO cells to produce cell line CHO-FSH 91 – producer rhFSH, which is used in production method rhFSH.

EFFECT: invention allows to increase production FSH human being for medical use.

4 cl, 9 dwg, 2 tbl, 7 ex

Similar patents RU2697273C1

Title	Year	Author	Number
EXPRESSION PLASMID FOR HUMAN RECOMBINANT FOLLICLE-STIMULATING HORMONE (FSH) IN CHO CELLS, EXPRESSION PLASMID FOR BETA-SUBUNIT OF HUMAN RECOMBINANT FSH IN CHO CELLS, CHO CELL PRODUCING HUMAN RECOMBINANT FSH, AND METHOD FOR PRODUCING ABOVE HORMONE	2013	Vorob'Ev Ivan Ivanovich Orlova Nadezhda Aleksandrovna Kovnir Sergej Vladimirovich Khodak Julija Aleksandrovna	RU2560596C2
CELL LINE HUFSHIK RELEASING RECOMBINANT HUMAN FOLLICLE-STIMULATING HORMONE	2012	Kirillova Irina Viktorovna Karjagina Tat'Jana Borisovna Orekhova Natal'Ja Evgen'Evna Gukasova Elena Aleksandrovna Pavlenko Daniil Mikhajlovich Polzikov Mikhail Aleksandrovich Bairamashvili Dmitrij Il'Ich	RU2502798C2
CELL LINE HUFSHKKC6, PRODUCER OF RECOMBINANT HUMAN FOLLICLE STIMULATING HORMONE (FSH) AND METHOD FOR PRODUCING FSH USING SAID LINE	2018	Zinchenko Aleksej Alekseevich Kalinina Elena Anatolevna Karyagina Tatyana Borisovna Miroshnikov Anatolij Ivanovich Pavlenko Daniil Mikhajlovich Stepanenko Vasilij Nikolaevich Sukhikh Gennadij Tikhonovich Chernyshov Sergej Vyacheslavovich Shibanova Elena Dmitrievna	RU2694598C1
Recombinant follicle-stimulating hormone (FSH), including alpha-2,3 - and alpha-2,6-sialation	2018	Cottingham Ian Plaksin Daniel White Richard Boyd	RU2745557C1
RECEPTOR OF HUMAN FOLLICLE-STIMULATING HORMONE (FSH), DNA, VECTOR, METHOD OF PREPARING, PHARMACEUTICAL COMPOSITION	1992	Kelton Kristi Ehnn Cheng Shirlej V'Ju Jen Nugent Norin Patris Shvajkkhardt Rene Linn	RU2193599C2
RECOMBINANT FOLLICLE-STIMULATING HORMONE (FSH) CONTAINING ALPHA-2,3- AND ALPHA-2,6-SYALILATION	2009	Cottingham Ian Plaksin Daniel White Richard Boyd	RU2682270C2
RECOMBINANT FOLLICLE-STIMULATING HORMONE (FSH) CONTAINING ALPHA-2, 3- AND ALPHA-2, 6-SYALILATION	2009	Kottingkhem, Ian Plaksin, Daniehl' Uajt, Richard Bojd	RU2537268C2
STRAIN OF CELLS OF CHINESE HAMSTER OVARIES - PRODUCER OF RECOMBINANT ANTIBODY AGAINST TUMOUR NECROSIS FACTOR ALPHA OF HUMAN BEING	2014	Ishchenko Aleksandr Mitrofanovich Kadykov Aleksandr Leonidovich Kalinin Jurij Tikhonovich Petrov Aleksandr Vladimirovich Pigareva Natal'Ja Vasil'Evna Simbirtsev Andrej Semenovich	RU2556816C1
SYNTHETIC DNA ENCODING THE ANTI-MULLERIAN HUMAN HORMONE, EXPRESSION VECTOR pTVK4pu/MISOPT THAT CONTAINS THIS DNA, CHINESE HAMSTER OVARY CHO-MIS CELLS STRAIN - RECOMBINANT ANTI-MULLERIAN HUMAN HORMONE PRODUCER	2016	Petrov Aleksandr Vladimirovich Karasev Maksim Mikhajlovich Kudling Tatyana Viktorovna Pigareva Natalya Vasilevna Sergeeva Valeriya Evgenevna Trofimov Aleksandr Viktorovich Ishchenko Aleksandr Mitrofanovich Gorbunov Aleksej Andreevich Vakhrushev Andrej Valentinovich Simbirtsev Andrej Semenovich	RU2616273C1
SYNTHETIC DNA ENCODING HUMAN ERYTHROPOIETIN, COMPRISING ITS VECTOR, METHOD OF PRODUCTION OF ERYTHROPOIETIN PRODUCER STRAIN, ERYTHROPOIETIN PRODUCER STRAIN	2014	Ishchenko Aleksandr Mitrofanovich Kadykov Aleksandr Leonidovich Petrov Aleksandr Vladimirovich Simbirtsev Andrej Semenovich	RU2548806C1

RU 2 697 273 C1

Authors

Monakhova Varvara Sergeevna

Klimov Nikolaj Anatolevich

Kudling Tatyana Viktorovna

Pigareva Natalya Vasilevna

Karasev Maksim Mikhajlovich

Shaldzhyan Aram Arutyunovich

Simbirtsev Andrej Semenovich

Dates

2019-08-13—Published

2019-02-22—Filed