COMPOSITION OF SOLUBLE MONOMERIC AND OLIGOMERIC FRAGMENTS OF PEPTIDOGLYCAN OF THE CELL WALL OF GRAM-NEGATIVE BACTERIA, METHODS FOR PRODUCTION AND APPLICATION THEREOF Russian patent published in 2022 - IPC C07K4/04 C07K1/14 C07K1/16 A61K38/01 A61P37/04 

FIELD: pharmaceuticals.

SUBSTANCE: described in the invention is a pharmaceutical composition including fragments of peptidoglycan of the cell wall of gram-negative bacteria, the main components whereof are oligomers of muramyldipeptides D, E, F, G, H with a molecular weight of 2,000 to 4,000 u, built from A, B and C blocks connected via glycosidic bonds, wherein muramylpeptide A is of β-N-acetyl-D-glucosaminyl-(1→4)-N-acetyl-D-muramyl-L-alanyl-D-isoglutamine-meso-diaminopimelic acid, muramylpeptide B is of β-N-acetyl-D-glucosaminyl-(1→4)-N-acetyl-D-muramyl-L-alanyl-D-isoglutamine-meso-diaminopimeloyl-D-alanine and muramylpeptide C constitutes a dimer of muramylpeptide B wherein the bond between the monomer residues of muramylpeptide B is executed by means of a carboxyl group of terminal D-alanine of one residue of B and the ω-amino group of the meso-diaminopimelic acid of another residue of B, wherein the trimeric muramyldipeptides D and E are formed due to the combination of the dimeric block C with the monomeric blocks B and A, respectively, the tetrameric muramylpeptides F and G are formed due to the combination of the dimeric block C with two monomeric blocks B or two monomeric blocks A, respectively, and the tetrameric muramylpeptide H formed due to the combination of the dimeric block C with the monomer blocks A and B. Also proposed is a method for producing the composition, including extraction of bacterial cells by a solution of a detergent at elevated temperature, dispersion of the resulting highly purified peptidoglycan under the impact of powerful ultrasound, followed by treatment with lysozyme.

EFFECT: described composition can be used in immunopharmacology, in particular, for creating supportive therapy agents intended for increasing the natural resistance of the body to infections of various natures.

11 cl, 7 dwg, 6 tbl