REAGENT SET FOR DETECTING RNA OF SARS-COV-2 VIRUS, CAUSATIVE AGENT OF NEW CORONAVIRUS DISEASE COVID-2019, BY REVERSE TRANSCRIPTION-POLYMERASE CHAIN REACTION IN REAL TIME Russian patent published in 2020 - IPC C12N15/11 C12Q1/6806 

FIELD: molecular biology; virology; biotechnology.

SUBSTANCE: invention relates to molecular biology, virology and biotechnology, namely to developing means of detecting and identifying agents of dangerous infectious diseases of viral etiology, and can be used to detect RNA of SARS-CoV-2 virus. Disclosed is a set of oligonucleotide primers and a fluorescent dye-labeled oligonucleotide probe for detecting SARS-CoV-2 virus RNA with the following structure: forward primer SARS-CoV-2_up: 5’-TTGAAGTTTAATCCACCTGCT-3’; reverse primer SARS-CoV-2_low: 5’-ACCGTTCAAGACTCTTTTGC-3’; oligonucleotide probe labeled with fluorescent dye: 5’-(R6G)-CTTATTACAGAGCAAGGGCTGGTGAAG-(RTQ2)-3’. Also disclosed is a reagent kit for detecting SARS-CoV-2 virus RNA by reverse transcription-polymerase chain reaction in real time, containing reverse transcriptase (MMLV-revertase), Tac-polymerase, an equimolar mixture of four deoxynucleotide triphosphates, an internal control sample, a negative control sample, a set of oligonucleotide primers and a fluorescent dye-labeled oligonucleotide probe according to cl.1, positive control sample in form of synthetic RNA, the sequence of which corresponds to hybridisation of oligonucleotide primers, wherein the oligonucleotide primers and the probe are used in a lyophilic dried state.

EFFECT: invention enables to perform reproducible detection of RNA virus SARS-CoV-2 in clinical biological samples with RNA concentration of not less than 1⋅10³ G-E in 1 cm³ without meeting the requirements of "cold chain" during transportation of set.

2 cl, 6 tbl, 4 ex