METHOD FOR THE DIAGNOSIS OF CRYPTOSPORIDIOSIS BY THE CONCENTRATION OF MOLECULAR MARKERS OF MICROORGANISMS IN THE BLOOD Russian patent published in 2022 - IPC G01N33/49 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to therapy, and can be used for the diagnosis of cryptosporidiosis. In the patient's venous blood, the concentration of the following molecular markers of microorganisms in nmol/g is determined by gas chromatography - mass spectrometry: decanoic acid, isopalmitic acid, 9,10-tetradecene acid, isolauric acid, 3-hydroxylauric acid, 2-hydroxylauric acid, isopentadecanoic acid, 9,10-pentadecenoic acid, iso-3-hydroxytridecanoic acid, iso-9,10-hexadecenoic acid, 7,8-hexadecenoic acid, 2-hydroxymyristic acid, 10-methylhexadecanoic acid, 3-hydroxyisopentadecanoic acid, antheisoheptadecanoic acid, cis-vaccenic acid, 9,10-heptadecenoic acid, lauric acid, isooctadecanoic acid, 3-hydroxy-palmitic acid, cyclononedecanoic acid, isoheptadecanoic aldehyde, cholestenediol, isoheptadecanoic acid, isoheptadecanoic acid, iso-heptadecanoic acid, iso-heptadecanoic acid, iso-heptadecanoic acid, iso-heptadecene aldehyde, 10-methyl-octadecanoic acid, 7,8-tetradecenoic acid, eicosenic acid, 3-hydroxyeicosanoic acid, 2-hydroxydocosanoic acid, 2-hydroxyhexacosylic acid. Then, using the classification equations of linear discriminant analysis, the values ​​of the diagnostic coefficients F1 and F2 are calculated, confirming the presence or absence of cryptosporidiosis. If F1 is greater than F2, the absence of cryptosporidiosis is diagnosed, and if F2 is greater than F1, cryptosporidiosis is diagnosed.

EFFECT: method provides the possibility of diagnosing cryptosporidiosis, which makes it possible to take into account the presence or absence of invasion, regardless of the biological form of the parasite, due to the non-invasive determination of the concentration of molecular markers of microorganisms in the blood by gas chromatography - mass spectrometry - and diagnostics using classification equations.

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