FIELD: medicine.
SUBSTANCE: invention relates to biomedicine, namely to increase of temperature stability and enzymatic stability of gelatine when making cell products. In particular, described is a method of increasing porosity, heat resistance and enzymatic stability of gelatine by modifying it with ribose and sodium chloride, which involves mixing 20 % gelatine solution with ribose to 4.5 % of ribose content and adding sodium chloride to the obtained mixture in ratio of 2:1, respectively, with further incubation of mixture for 72 hours at temperature of 50 °C and washing in distilled water for 48 hours.
EFFECT: increased enzyme resistance and thermal stability of the scaffold while maintaining its 3D architectonics using an inexpensive and simple technology.
1 cl, 1 dwg, 2 tbl
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD FOR INCREASING HEAT RESISTANCE OF GELATINE WHEN MODIFYING IT WITH GLUTARALDEHYDE | 2018 |
|
RU2687559C1 |
| METHOD FOR INCREASING THE HEAT RESISTANCE OF GELATIN BY MODIFICATION OF IT WITH DIHYDROQUERCETIN AND TEMPERATURE | 2017 |
|
RU2664445C1 |
| METHOD OF INCREASING THE HEAT RESISTANCE OF GELATIN BY MODIFICATION WITH FLAVONOIDS | 2017 |
|
RU2664440C1 |
| DEVICE AND METHOD FOR FABRICATION OF 3D FABRIC ENGINEERING STRUCTURES FOR CULTIVATION OF CELLS | 2019 |
|
RU2729415C1 |
| COMPOSITION FOR FORMATION OF MACROPOROUS MEDIA USED IN VOLUME CULTURE OF ANIMAL OR HUMAN CELLS AND METHOD FOR PRODUCTION OF SAID CARRIER | 2015 |
|
RU2594427C1 |
| METHOD FOR TEXTILE TREATMENT FOR CARDIOVASCULAR SURGERY | 2011 |
|
RU2470671C1 |
| SURGICAL GLUE "BIOKLEY-LAB" | 2002 |
|
RU2236260C2 |
| METHOD FOR PRE-IMPLANTATION PROCESSING OF TEXTILE PRODUCTS FOR CARDIOVASCULAR SURGERY | 2021 |
|
RU2794740C2 |
| BIOCATALYST AND METHOD FOR ITS PREPARING | 2002 |
|
RU2233327C2 |
| METHOD FOR FORMATION OF BIORESORBABLE FIBROIN FILMS USING METACRYLATED GELATINE | 2016 |
|
RU2645200C1 |
