RECOMBINANT VECTOR CONTAINING THE IMMUNOGENIC PROTEIN OF THE AFRICAN SWINE FEVER VIRUS, RECOMBINANT BACTERIA AND THEIR APPLICATION Russian patent published in 2022 - IPC C12N15/74 C12N15/35 A61K39/12 A61P31/20 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, namely genetic recombination. A recombinant vector containing a polynucleotide encoding the immunogenic protein of the African swine fever virus is proposed, where the expression vector of Lactobacillus pVE5523 is taken as the basis of the recombinant expression vector, and the nucleotide sequence encoding the immunogenic protein of the African swine fever virus is cloned between the restriction sites EcoFN and Sa/I of the vector taken as the basis. The immunogenic protein of the African swine fever virus contains protein p72 and protein p54 of the African swine fever virus of the Chinese strain Jilin. Recombinant Lactobacillus expressing the immunogenic protein of the African swine fever virus contains a recombinant expression vector. The method for constructing recombinant Lactobacillus includes: (1) cloning of the nucleotide sequence encoding the p72 protein of the African swine fever virus and the nucleotide sequence encoding the p54 protein of the African swine fever virus into the expression vector of Lactobacillus pVE5523 for the construction of recombinant plasmids pVE5523-ASFV-p72 and pVE5523-ASFV-p54, respectively; (2) transformation of competent Lactobacillus cells by recombinant plasmids pVE5523-ASFV-p72 and pVE5523-ASFV-p54 to produce recombinant Lactobacillus expressing the p72 protein of the African swine fever virus and recombinant Lactobacillus expressing the p54 protein of the African swine fever virus, respectively. The use of a recombinant expression vector or recombinant Lactobacillus for the prevention and/or treatment of African swine fever or for the manufacture of medicines for the prevention and/or treatment of African swine fever has been proposed. Oral preparation of live bacteria for the prevention of African swine fever infection, where the active ingredients of the oral preparation of live bacteria contain recombinant Lactobacillus expressing the protein p72 of the African swine fever virus, and recombinant Lactobacillus expressing the protein p54 of the African swine fever virus, designed by the construction method. The method for treating African swine fever includes: taking an oral preparation of live bacteria by animals, where the amount taken is 5 ml per animal.

EFFECT: invention provides safe, effective and fast prevention of infection of pigs with the African swine fever virus and does not include the immune process.

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