METHOD FOR OBTAINING FICIN AND CHITOSAN ACETATE HYBRID PREPARATION IN THE FORM OF A DENSE SOLUTION Russian patent published in 2023 - IPC C12N11/02 C12N9/50 A61K38/48 

FIELD: biotechnology.

SUBSTANCE: method for producing a hybrid preparation of ficin and chitosan acetate in the form of a thick solution is proposed, including the immobilization of the enzyme preparation of ficin in a thick solution of chitosan acetate with a molecular weight of 600 kDa in the ratio of 10 ml of ficin solution at a concentration of 2 mg/ml per 1 g of dry chitosan acetate, incubation at room temperature for 2 hours with constant stirring at 250 rpm; 0.05 M glycine buffer with pH 10.0 is used as a buffer solution for immobilization. The resulting preparation is washed by dialysis with 50 mm Tris-HCl buffer pH 7.5.

EFFECT: extension of the arsenal of methods for obtaining stabilized ficin preparations used for medical purposes.

1 cl, 2 dwg, 1 ex