METHOD FOR MODELING CHICKEN MICROBIOTA IN AN ARTIFICIAL CAECUM Russian patent published in 2022 - IPC G09B23/28 C12N1/20 C12Q1/02 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to experimental medicine, and can be used to simulate the microbiota of the chicken cecum in artificial intestinal systems. First, into a flask containing a nutrient medium composition with the ratio of ingredients, g/l: starch - 0.5-2.5 g, isolated soy protein - 10-20 g, unrefined sunflower oil - 10-30 ml, MgSO₄ - 0.25-1.5 g, NaCl - 3-6 g, K₂HPO₄ - 0.25-1.5 g, MnSO₄ - 0.05-0.06 g, FeSO₄ - 0.05-0.06 g, Tween 80 - 0.5-1.5 ml, water up to 1 l, a defrosted starter is introduced from the contents of the caecum of chickens containing microorganisms, and incubated in an anaerobic chamber under conditions corresponding to the simulated area of ​​the intestine. Then the test sample is placed in the reactor - a model of artificial chicken intestine, while setting the temperature and gas conditions corresponding to the specified conditions.

EFFECT: method provides an opportunity to simulate the microbiota of the chicken caecum in artificial systems by developing a model of the artificial chicken intestine, including the composition of the artificial intestinal medium, where a “starter” is added containing microbial cultures that normally colonize the chicken digestive tract, and a nutrient medium for the growth of the microbiota, which makes it possible to take into account the influence of microbiota in the systems of the artificial digestive tract of chicken, to evaluate the effect of additives on the intestinal microbiota, and to screen the properties of probiotic strains.

1 cl, 2 ex, 4 dwg