FLUORESCENT MULTI-PURPOSE NANOSCALE LABEL AND CONJUGATES BASED THEREON Russian patent published in 2022 - IPC C09K11/54 B82B3/00 C09K11/56 B82Y15/00 B82Y30/00 G01N21/64 G01N33/533 

FIELD: nanotechnology.

SUBSTANCE: invention relates to nanotechnology, optoelectronics, and medical diagnostics, and can be used in specific fluorescent molecular-targeted marker imaging, diagnosis of diseases, and in manufacturing leak detection apparatuses. Quantum dot has a wurtzite crystal structure and a size of 6 to 8 nm. The core of the quantum dot has a wurtzite crystal structure, a size of 2 to 4 nm, and a composition CdTeSeS; and the shell, a composition CDs, CdZnS, CDs/ZnS, CDs/CdZnS, or CdZnS/ZnS. There are 5 to 8 monolayers of the shell. The quantum dot is characterised by the following molar content of Cd, Se, and S, %: 21 to 29, 14 to 20, 52 to 64, respectively; a quantum efficiency of 50 to 70% and a high fluorescence intensity in the range of 630 to 720 nm. The fluorescent conjugate for molecular marker imaging consists of said quantum dot coated with a polymer shell and a ligand ensuring the binding specificity. The polymer shell is made of a copolymer of methoxy polyethylene glycol acrylate-co-maleic anhydride-co-ethylene glycol diacrylate terminated with 1,6-hexanedithiol; a copolymer of methoxy polyethylene glycol acrylate-co-maleic anhydride-co-ethylene glycol diacrylate with a thiolised oligomer; a copolymer of polyvinylpyrrolidone and acrylic acid. The ligand is selected from folic acid, an antibody or a fragment thereof, and conjugated with the polymer shell via a linker constituting polypropylene glycol, polyethylene glycol acrylate, or streptavidin. Molecular marker constitutes a substance of a protein or nucleic nature, wherein said substance is a metabolic byproduct of living cells and is included therein. Said conjugates are also characterised by a high fluorescence intensity.

EFFECT: creation of nanoscale label applicable in medical diagnostics.

12 cl, 32 dwg, 1 tbl