FIELD: medicine.
SUBSTANCE: invention relates to cell biology and medicine, in particular to clinical pharmacology, and reveals a method for inhibiting the glycoprotein-P transporter protein in an in vitro experiment. To implement this method, a human colon adenocarcinoma cell line (Caco-2) is cultured in a Dulbecco modified Igla medium with a glucose content of 4500 mg/l for 21 days. This medium also contains L-glutamine 4 mM, 15% bovine serum, 100 U/ml of penicillin and 100 mcg/ml of streptomycin. After that, DL-buthionine sulfoximine is added to the medium until its concentration in the medium of 50, 100 or 500 mcM is reached and incubated for 3 hours.
EFFECT: present invention makes it possible to implement a simple and affordable method for inhibiting the glycoprotein-P transporter protein in an in vitro experiment.
1 cl, 1 tbl, 1 ex
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