SYSTEM FOR CELL CULTURING Russian patent published in 2017 - IPC C12M3/02 C12Q1/18 C12N5/71 C12N1/14 C12N1/20 

FIELD: biochemistry.

SUBSTANCE: group of inventions relates to biochemistry. Cell culturing system, system for assessing intestinal effector agents, containing cell culturing system, are disclosed, methods of cell culturing, production of intestinal organoid and evaluation of treating of intestinal effector agents are also disclosed. Cell culturing system comprises device with liquid channel, liquid source, membrane in channel, intestinal epithelial cells on surface of membrane and bacterial cells adhering to intestinal cells. Method of cells culturing involves providing device with intestinal epithelial cells and bacterial cells, equipment it with culture medium at flow rate sufficient for removal of organic acids and unbound bacterial cells. Method for production of intestinal organoid includes delivery of cultural medium to system and cultivation of epithelial cells of intestine and bacterial cells in vitro. Evaluation method of treating intestine involves contact of intestinal epithelial cells or bacterial cells in system with potential intestinal effector agent and measurement of cell response.

EFFECT: invention provides development in vitro model simulating mechanical, structural, absorbent, transport and pathophysiological properties of intestine.

41 cl, 23 dwg, 1 tbl