STRAIN “PRRS-1SBC” OF THE SWINE REPRODUCTIVE AND RESPIRATORY SYNDROME VIRUS FOR THE MANUFACTURE OF DIAGNOSTIC AND VACCINE PREPARATIONS, A VACCINE AGAINST SWINE REPRODUCTIVE AND RESPIRATORY SYNDROME AND A METHOD FOR OBTAINING A VACCINE AGAINST SWINE REPRODUCTIVE AND RESPIRATORY SYNDROME (VARIANTS) Russian patent published in 2022 - IPC C12N7/00 

FIELD: veterinary medicine.

SUBSTANCE: invention relates to the field of veterinary medicine, veterinary biotechnology and concerns the production of preventive and diagnostic drugs. A strain of swine reproductive and respiratory syndrome virus for the manufacture of diagnostic and vaccine preparations belonging to the genus Arterivirus, family Arteriviridae, order Nidovirales deposited at the State Collection of Viruses of the Institute of Virology of the Federal State-Financed Entity “Honorary Academician N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology” under No. 2928 is presented. A vaccine for the prevention of swine reproductive and respiratory syndrome containing an attenuated strain of swine reproductive and respiratory syndrome virus with a titer of 4.0-7.5 lg TCD50/cm³ and a mixture of sucrose, peptone and gelatose is presented. Vaccine variants are presented where a mixture of sucrose, peptone, gelatose and tris-HCl buffer is used as a stabilizer, or a mixture of sucrose, peptone, gelatose, tris-HCl buffer, ethylenediaminetetraacetic acid is used as a stabilizer, or a mixture of sucrose, peptone, gelatose, tris-HCl buffer, ethylenediaminetetraacetic acid and chitosan is used as a stabilizer. A method for obtaining a vaccine for the prevention of swine reproductive and respiratory syndrome is proposed, including infection of the transplanted cell culture with virus-containing material from an attenuated strain of the swine reproductive and respiratory syndrome virus, reproduction of the virus in the transplanted cell culture, collection of virus-containing material followed by obtaining an immunizing antigen of the swine reproductive and respiratory syndrome virus and adding a stabilizer to it, an attenuated strain of the swine reproductive and respiratory syndrome virus with a titer of 4.0-7.5 lg TCD50/cm³ is used as a virus-containing material, and MA-104 cells, or Vero cells, or Marc-145 cells, or CV1 cells are used as a transferable cell culture, and virus reproduction is carried out at a temperature of 36-38°C for 72-120 hours at a pH of 7.2-7.4, and the immunizing antigen of the swine reproductive and respiratory syndrome virus is obtained by 1-2-fold freezing and thawing of infected cells, followed by centrifugation at 1000-2000 thousand. rpm for 15-20 minutes and separation of the supernatant, and the vaccine is obtained by adding a stabilizer to the supernatant.

EFFECT: invention makes it possible to increase the immunogenicity of the target product during long-term storage of the target product.

6 cl, 1 tbl