FIELD: biotechnology.
SUBSTANCE: invention relates to the field of biotechnology, in particular to a method for editing the GJB2 gene based on the CRISPR-Cas system to correct the variant c.del35G in a human cell cultured in vitro, excluding human germline cells.
EFFECT: invention is effective for correcting a section of the human genome smaller than 120 nucleotides carrying the c.del35G mutation in the human GJB2 gene to a variant of the healthy human genome with a synonymous replacement of C.G30C in the GJB2 gene in cells cultured in vitro.
1 cl, 1 tbl, 1 ex, 2 dwg
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