PDE6B GENE KNOCKOUT MOUSE MODEL CREATING AND APPLYING METHOD Russian patent published in 2023 - IPC A01K67/27 

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, in particular to a method for creating a genetically engineered mouse model of pigmented autosomal recessive retinitis having a deletion of two nucleotides in the second exon of the pde6b gene, which encodes the β-subunit of cyclic guanidine monphosphate phosphodiesterase, by disabling the pde6b gene. Method includes a guide RNA mixture microinjection, which is complementary to the pde6b gene second exon, capable of forming a complex with the effector protein Cas9 and hybridizing with the target sequence of the pde6b gene, and the Cas9 protein into a mouse zygote, setting the zygotes after microinjection into the uterus of a pseudo pregnant female mouse, genotoping the resulting mice, and breeding a line of mice that are homozygous due to the pde6b gene knockout.

EFFECT: invention provides for efficiently obtaining a directed deletion of 2 nucleotides in the second exon of the pde6b gene leading to a reading frame shift in the translation of the gene and gene disabling.

1 cl, 3 dwg