FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, in particular to a method for producing a rabbit with gene knockout CSN2 (β-cas). Said method involves editing the CSN2 (β-cas) by CRISPR/Cas9 method, for which zygotes, assessed as normal, are subjected to microinjection procedure with genetic construct, which is a mixture of sgRNA and CRISPR/Cas9 enzyme in ratio 1:1, living embryos are separated from dead ones as a result of microinjection and embryos are transplanted to female recipients.
EFFECT: present invention enables to obtain a rabbit with a CSN2 (β-cas) gene knockout, capable of secreting rabbit milk without β-casein.
1 cl, 3 dwg, 5 ex
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