METHOD OF DECELLULARIZATION OF A CARDIOVASCULAR SYSTEM HOMOGRAFT WITH SUPERCRITICAL CARBON DIOXIDE Russian patent published in 2023 - IPC A61F2/07 A61F2/24 

FIELD: medicine; cardiac surgery.

SUBSTANCE: invention relates to a method of creating biological prostheses, namely homografts. The following is proposed: a method of decellularization of the homograft of the cardiovascular system by hybrid processing with preconditioning in a solution of detergents, washing in distilled water and subsequent extraction in supercritical CO₂ under the following thermostatically controlled conditions: 37±0.2°C, a pressure of 25 MPa and a flow rate of 3±0.5 ml/min with the placement of a wet aortic root in a high-pressure reactor, filling the CO₂ chamber at a pressure of 6–7 MPa, then turning on the thermostat, setting and maintaining the medium at a temperature of 37°C and pressure of 25 MPa. As a decellularizing agent, supercritical carbon dioxide, a section of the outflow tract of the right or left ventricle of the heart, the mitral or aortic valve of the heart, or a fragment of the aorta is used, the component is taken while maintaining the non-functional area adjacent to it, designed to ensure, during the implementation of the method, non-contact with the component and ready-made homograft, manipulations by capturing this area with tweezers, decellularization is carried out no later than 4–6 hours after the removal of valve-containing fragments of the aorta using glass containers with a volume of 250 ml and an orbital shaker, before decellularization, the aortic homograft is washed with a solution of povidone-iodine, the isolated valves are preconditioned in detergent solution of 0.5 vol.% of sodium dodecyl sulfate and 0.5 vol.% of sodium deoxycholate for 24 hours and washed in distilled water for 12 hours at room temperature under sterile conditions and constant stirring at a speed of 200 rpm, decellularization is carried out under the following thermostatic conditions: 37±0.2°C, a pressure of 25 MPa and a flow rate of 3±0.5 ml/min with the placement of a wet aortic root in a high-pressure reactor, and subsequent filling of the CO₂ chamber at a pressure of 6–7 MPa, then turning on the thermostat, setting the temperature to 37°C and a pressure to 25 MPa, using a fine adjustment valve, setting the flow rate to 3 ml/min, and under such conditions, the samples are kept for 3 hours, after which the thermostat is turned off and the pressure is slowly reduced over 30 minutes.

EFFECT: invention provides the possibility of simultaneous decellularization and sterilization of the homograft of the cardiovascular system, as well as increasing its service life.

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