ANTICANCER DRUG IN FORM OF CAPE-LOADED MICRO-VESICLES OF DIRECTED ACTION AND ITS DEVELOPMENT METHOD Russian patent published in 2022 - IPC A61K31/216 A61K35/36 A61P35/00 

FIELD: medicine; pharmaceutics.

SUBSTANCE: group of inventions relates to the field of medicine and pharmaceutics, namely to a drug purposefully acting on SH-SY5Y neuroblastoma and to a method for the production of an anticancer drug in the form of CAPE-loaded micro-vesicles, purposefully acting on SH-SY5Y neuroblastoma. The drug purposefully acting on SH-SY5Y neuroblastoma is obtained by loading with caffeic acid phenethyl ester (hereinafter – CAPE) drug of cellular micro-vesicles, which are formed by cultivation of skin stem cells in Dulbecco’s modified eagle medium (hereinafter – DMEM medium) containing 10% embryonic bovine serum and 1% of PSA at a temperature of 37°C and 5% of carbon dioxide, followed by specialization by administration of a neurobasal solution containing 10 ng/ml of bFGF, 10 ng/ml of EGF, 1% B7 additive, 1% of insulin, transferrin, and selenium (hereinafter – ITS), 10% glutamine, and 1% of PSA once every two days for 12-14 days. The method for the production of an anticancer drug in the form of CAPE-loaded micro-vesicles, purposefully acting on SH-SY5Y neuroblastoma, includes stages of: cultivation of skin stem cells in DMEM medium containing 10% embryonic bovine serum and 1% of PSA at a temperature of 37°C and 5% of carbon dioxide; implementation of a specialization protocol, which provides that cells in the culture medium, which have achieved sufficient confluence of 70-80%, are seeded into cell culture tablets, the neurobasal solution containing 10 ng/ml of bFGF, 10 ng/ml of EGF, 1% B7 additive, 1% of ITS, 10% glutamine, and 1% of PSA is administered once every two days for 12-14 days; preparation of an initial solution by dissolution of CAPE in dimethyl sulfoxide (DMSO); isolation of micro-vesicles from specialized skin cells; addition of CAPE to micro-vesicles; separation of CAPE-loaded micro-vesicles from CAPE, which is present in the solution in a free state and is not loaded into the micro-vesicle; preparation of an anticancer drug in the form of CAPE-loaded micro-vesicles purposefully acting on SH-SY5Y neuroblastoma, which is the final product.

EFFECT: above-mentioned group of inventions allows for the use of a drug in the form of CAPE-loaded micro-vesicles, purposefully acting on SH-SY5Y neuroblastoma, specific for this type of cells, in much lower concentrations, as well as for minimization of inflammation and toxicity to the body.

13 cl, 2 dwg