SUPPRESSION OF EXPRESSION OF GENE C-KIT IN CELLS OF NEUROBLASTOMA AND LENTIVIRAL CONSTRUCTIONS, DIRECTING SYNTHESIS SHPHK, SPECIFIC FOR GIVEN GENE Russian patent published in 2017 - IPC C12N15/113 C12N15/63 

FIELD: biotechnologies.

SUBSTANCE: recombinant genetic construction cloned into retroviral or lentiviral vector according to the sites restriction XpaI and XhoI and directed to the suppression of the expression of oncogene C-KIT in neuroblastoma cells, and the use of said construction for the regulation of C-KIT gene activity and its protein product are suggested. A recombinant genetic construction is a kit-2-sense strand (5'-p-aacgCGGTTGAATGTAAGGCTTActtcctgcaaTAAGCCTTACATTCAACCGTGtttttc-3'), kit-2-antisense strand (5'-p-tcgagaaaaaCACGGTTGAATGTAAGGCTTAttgcaggaagTAAGCCTTACATTCAACCGcgtt-3'). A recombinant genetic construction may be used to suppress the expression of gene C-KIT in neuroblastoma cells and regulation of C-KIT gene activity and its protein product.

EFFECT: downregulation of the expression of oncogene C-KIT when introducing it in neuroblastoma cells expressing C-KIT, and slowing the growth rate of cancer cells.

2 cl, 4 dwg, 2 ex