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2 786 213

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IPC

C12Q1/68(2006-01-01)

A61K39/395(2006-01-01)

(21) (22)

Application

2022122746, 2022-08-23

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Start date

2022-08-23

(22)

Actual filing date

2022-08-23

(45)

Published

2022-12-19

(72)

Inventor

Sprygin Aleksandr VladimirovichKrotova Alena OlegovnaMazlum AliShalina Kseniya AleksandrovnaPrutnikov Pavel VladimirovichNesterov Aleksandr AleksandrovichShumilova Irina NikolaevnaDoronin Maksim IgorevichByadovskaya Olga Petrovna

(73)

Holder

Federalnoe Gosudarstvennoe Byudzhetnoe Uchrezhdenie Tsentr Okhrany Zdorovya Zhivotnykh"

METHOD FOR DIFFERENTIATING THE GENOME OF THE NISHI VACCINE STRAIN FROM FIELD ISOLATES OF SHEEP POX VIRUS BY REAL-TIME POLYMERASE CHAIN REACTION WITH HIGH-RESOLUTION PEAK ANALYSIS Russian patent published in 2022 - IPC C12Q1/68 A61K39/395

Abstract RU 2786213 C1

FIELD: biotechnology.

SUBSTANCE: invention relates to biotechnology, to molecular diagnostic tools, namely to differentiation of the genome of the NISHI vaccine strain from field isolates of sheep pox virus by real-time polymerase chain reaction with high-resolution peak analysis. The developed method is characterized by an analytical specificity of 100%. In the 95% confidence interval, the diagnostic sensitivity for this method is 97.49-100.00%, the diagnostic specificity is 97.20-100.00%.

EFFECT: invention provides the ability to differentiate the genome of the NISHI vaccine strain from field isolates of sheep pox virus in a short period of time (no more than 3 hours) by real-time polymerase chain reaction with high-resolution peak analysis.

3 cl, 4 dwg, 7 tbl, 5 ex

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RU 2 786 213 C1

Authors

Sprygin Aleksandr Vladimirovich

Krotova Alena Olegovna

Mazlum Ali

Shalina Kseniya Aleksandrovna

Prutnikov Pavel Vladimirovich

Nesterov Aleksandr Aleksandrovich

Shumilova Irina Nikolaevna

Doronin Maksim Igorevich

Byadovskaya Olga Petrovna

Dates

2022-12-19—Published

2022-08-23—Filed