METHOD FOR PREDICTING WHEAT PRODUCTIVITY BASED ON CHLOROPHYLL FLUORESCENCE INDICES Russian patent published in 2023 - IPC A01G7/00 A01G22/20 

FIELD: plant breeding.

SUBSTANCE: invention relates to the field of plant breeding, in particular to the prediction of wheat productivity based on chlorophyll fluorescence in the early stages of its development. The method involves placing two-week-old wheat seedlings of various varieties into a fluorescence recording system based on the PAM fluorimetry method. They are adapted to darkness for at least 10-15 minutes, the dark yield of chlorophyll F₀ fluorescence and the maximum dark yield of chlorophyll F_m fluorescence are measured after dark adaptation for at least 10-15 minutes. The actinic light is turned on and the current level of chlorophyll F fluorescence is recorded, as well as the parameter Fm' using saturating flashes applied at intervals of 10-30 seconds for at least 10-15 minutes. The F_PSII indicator is calculated according to the formula F_PSII = (F_m' − F)/F_m', where F_PSII is the quantum yield of photochemical reactions of photosystem II, Fm' is the maximum fluorescence yield, and F is the current fluorescence yield. The obtained light-induced dynamics of the F_PSII indicator is analyzed and the values of the indicators are determined, such as the effective quantum yield of photoreactions of photosystem II in the state adapted to actinic light F_PSIIef and the half-time F_PSII to reach the stationary level after turning on the actinic light t_1/2(F_PSIIef) for each variety. All the studied plant variants are ranked first by the value of F_PSIIef and the ones with the highest values of this indicator among the studied plant varieties are selected, then the selected plant varieties are ranked by the value of t_1/2(F_PSIIef) and the options with the lowest values of this indicator are selected as promising and potentially high productivity.

EFFECT: method provides the possibility of predicting the potential productivity of wheat varieties and speeding up breeding.

6 cl, 7 dwg, 1 tbl, 2 ex