METHOD FOR NON-DESTRUCTIVE DIAGNOSTICS OF PLANT FUNCTIONAL STATUS EX VITRO AND IN VITRO Russian patent published in 2019 - IPC A01G7/04 

FIELD: agriculture; forestry.

SUBSTANCE: present invention relates to experimental biology, plant growing, agriculture and forestry. Method involves measuring light-scattering dynamics of photosynthesizing plant tissue in the process of illumination with monochromatic optical radiation of the blue region of the spectrum in the zone of the first absorption maximum of chlorophyll 460–480 nm. Record of chlorophyll fluorescence intensity drop of leaf area 2 mm is recorded² during first illumination with monochromatic optical radiation of blue spectrum region with power density higher than photosynthesis saturation of 600–900 W/m² for 30–180 s. Then, illumination is switched off for 30–60 s and after this dark recovery pause in the same zone of object measurements light intensity decrease again during second illumination is registered with monochromatic optical radiation of blue area of spectrum of the same power density during 30–180 s. Photosynthetic activity and resistance to photoinhibition are evaluated by the values of K_PSA= (I¹_swing-I¹_cm) I¹_swing and K_UVR= (I²_swing-I²_cm)/(I¹_swing-I¹_cm), where I¹_swing is maximum intensity of fluorescence of chlorophyll per 1–2 s of first exposure; I¹_cm - steady-state level of fluorescence intensity of chlorophyll for 30–180 s of first exposure; I²_swing - maximum fluorescence intensity of chlorophyll for 1–2 s of second illumination; I²_cm - stationary level of fluorescence intensity of chlorophyll by 30–180 s of second illumination. At that, higher values of K_FSA and K_UVR, the better the functional state of plants.

EFFECT: method provides higher efficiency and reduced labour intensity of determining the functional state of plants ex vitro and in vitro.

1 cl, 1 dwg, 1 tbl, 1 ex