OLIGONUCLEOTIDES FOR DETECTION OF 8 SEROTYPE OF STREPTOCOCCUS PNEUMONIAE Russian patent published in 2023 - IPC C12Q1/6806 C12Q1/6876 C12Q1/689 

FIELD: biotechnology.

SUBSTANCE: invention relates to the diagnostic oligonucleotides, and can be used in medicine to identify pathogens of pneumococcal infections (PI) and evaluate the effectiveness of ongoing immunoprophylactic measures. Proposed set including forward primer 8F, reverse primer 8R and fluorescent probe 8Z, to determine the 8th serotype of Streptococcus pneumoniae by real-time polymerase chain reaction (RTP-PCR). Forward 8F and reverse 8R primers are complementary to a region of serotype 8 S. pneumoniae gene that does not have homology with fragments of the cps locus of representatives of other S. pneumoniae serotypes, and 8Z fluorescent probe is an oligonucleotide containing a fluorophore and a fluorescence quencher, which makes it possible to detect the amplified fragment.

EFFECT: oligonucleotides do not cross-react with other tested S. pneumoniae serotypes, amplify a region conserved for the 12FAB serotype with a specificity of 8%, and make it possible to determine the presence or absence of a serotype-specific target for 12FAB serotype S. pneumoniae in biological material samples.

3 cl, 3 dwg, 1 tbl, 5 ex