FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, specifically to a method of producing recombinant nuclease preparations of the Cas family of the CRISPR/Cas system in strain cells Escherichia coli Rosetta-gami B (DE3) and further purification thereof.
EFFECT: disclosed method, including a step for further purification of preparations using detergent Triton X-114, reduces content of bacterial endotoxins to 0,3–1,5 EU/ml in the obtained preparations.
21 cl, 25 dwg, 4 tbl, 5 ex
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