FIELD: oligosaccharides.
SUBSTANCE: invention relates to a method of purifying sialylated oligosaccharides obtained by microbiological fermentation comprising the following steps: 1) separating the biomass from the fermentation broth; 2) removal of cations from the fermentation broth; 3) removal of anionic impurities from the fermentation broth; and 4) removing compounds having a molecular weight lower than the molecular weight of the sialylated oligosaccharide to be purified from the fermentation broth, wherein the method further comprises an electrodialysis step, wherein (i) acidic conditions are used to conduct the electrodialysis or (ii) the electrodialysis is carried out under neutral conditions with using bipolar membranes, and wherein the sialylated oligosaccharide is a sialylated human milk oligosaccharide selected from the group consisting of 3'-SL, 6'-SL, LST-a, LST-b, LST-c, 3-F-SL, DS-LNT and FLST-b.
EFFECT: increasing the purity of sialylated oligosaccharides obtained by microbiological fermentation.
13 cl, 8 dwg, 3 tbl, 5 ex
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