METHOD FOR PREPARING PREPARATION OF RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas AND PREPARATION FOR DETECTING ANTIBIOTIC RESISTANCE GENE bla-TEM-1B IN ULTRA-LOW CONCENTRATIONS Russian patent published in 2024 - IPC C12N15/11 C12N15/113 C12N9/22 C12P21/02 C12Q1/6876 C12Q1/6888 

FIELD: biotechnology.

SUBSTANCE: described is a group of inventions comprising a method of producing a preparation of a ribonucleoprotein complex CRISPR-Cas and a preparation of the ribonucleoprotein complex CRISPR-Cas for detecting the antibiotic resistance gene bla-TEM-1B, obtained by said method. Method comprises steps for synthesis of guide RNA with SEQ ID NO: 1-6, combining Cas-protein of the family CRISPR-Cas LbCpf1 from Lachnospiraceae into a complex with at least one guide RNA and lyophilic drying of the ribonucleoprotein complex CRISPR-Cas, thus obtaining a ribonucleoprotein complex preparation CRISPR-Cas.

EFFECT: invention extends the range of products for detecting the antibiotic resistance gene bla-TEM-1B.

6 cl, 10 dwg, 4 tbl, 5 ex