FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, particularly to a method of producing a preparation of a ribonucleoprotein complex CRISPR-Cas. Said method comprises the following steps: (i) combining Cas-protein of CRISPR-Cas LbCpf1 family from Lachnospiraceae into complex with at least one guide RNA with SEQ ID NO: 1-8; and optionally (ii) freeze-drying said complex. In addition, the invention also relates to a preparation of the ribonucleoprotein CRISPR-Cas complex for detecting the bla-NDM-1 antibiotic resistance gene obtained using said method.
EFFECT: present invention provides considerable improvement of efficiency of detection of antibiotic resistance gene bla-NDM-1.
4 cl, 13 dwg, 4 tbl, 5 ex
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