METHOD OF CULTIVATING A PRIMARY MIXED CULTURE OF NEURONS FOR ASSESSING MITOCHONDRIAL RESPIRATION Russian patent published in 2023 - IPC C12N5/793 A61K31/195 

FIELD: molecular cell physiology and biochemistry.

SUBSTANCE: invention can be used in clinical pharmacology to test the degree of influence of neurotoxins or neuroprotectors on the intensity of various parts of cellular respiration. The method includes obtaining a primary culture of hippocampal neurons from an 18-day-old embryo or hippocampus, midbrain or cerebral cortex of a small rodent by trypsinization, seeding cells in pre-treated culture plates, incubating cells using high- and low-serum neurobasal culture media, measuring parameters of basic mitochondrial respiration on days 2, 5, 8 and 11 of culture differentiation. The high-serum medium contains 10% of fetal calf serum, 2% of B-27 protein supplement, 0.5 mM of L-Glutamax, 1% of PenStrep, and low-serum medium contains 0.5% of fetal calf serum, 2% of B-27 protein supplement, 1% of L-Glutamax, and 1% of PenStrep.

EFFECT: obtaining a method of cultivating a primary mixed culture of neurons for assessing mitochondrial respiration.

3 cl, 4 dwg, 1 tbl, 5 ex