FIELD: biotechnology.
SUBSTANCE: nucleic acid construct for expression of the CRM197 protein is proposed, containing a nucleic acid encoding a signal sequence that contains the sequence SEQ ID NO: 6–7, 8–10 and SEQ ID NO: 8 and the CRM197 protein gene. An expression vector for expression of the CRM197 protein containing the specified nucleic acid construct, a recombinant microorganism for the expression of the CRM197 protein containing the specified nucleic acid construct or the specified expression vector, as well as a method for producing the CRM197 protein are also proposed.
EFFECT: invention ensures efficient expression of CRM197 protein and efficient secretion of CRM197 protein into the periplasm without changing the pH of the medium.
9 cl, 18 dwg, 8 tbl, 5 ex
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