FIELD: biotechnology.
SUBSTANCE: following is presented: a method that is implemented by extracting DNA from a sample of biological material, conducting a real-time PCR reaction using a set of oligonucleotide primers and a probe for detecting human herpes virus 6A of the following SEQ ID NO: 1–3 and DNA calibrators with a known concentration, amplification, analysis and interpretation of the results by constructing a calibration line and determining the concentration of target DNA in the test sample. Also a set of oligonucleotide primers and a probe are presented, the said probe has the following nucleotide composition: forward primer HHV-6A/B-for - the following SEQ ID NO: 1; reverse primer HHV-6A/B-rev – the following SEQ ID NO: 2; fluorescent probe HHV-6B-probe – the following SEQ ID NO: 3.
EFFECT: invention can be used in clinical practice and allows to perform 100% specific identification of human herpes virus 6A (HHV-6A) with simultaneous quantitative assessment of the result obtained.
10 cl, 3 tbl, 3 ex
| Title | Year | Author | Number |
|---|---|---|---|
| METHOD OF QUANTITATIVE DETERMINATION OF DNA OF HUMAN HERPES VIRUS 6A (HHV-6A) AND DNA OF HUMAN HERPES VIRUS 6B (HHV-6B) AND A SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR ITS IMPLEMENTATION | 2022 |
|
RU2800731C1 |
| METHOD OF QUANTITATIVE DETERMINATION OF DNA OF HUMAN HERPES VIRUS 6A (HHV-6A) AND DNA OF HUMAN HERPES VIRUS 6B (HHV-6B) AND A SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR ITS IMPLEMENTATION | 2022 |
|
RU2800995C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR SPECIES DIFFERENTIATION OF HUMAN HERPES VIRUS 6A AND HUMAN HERPES VIRUS 6B AND METHOD OF ITS USE | 2022 |
|
RU2806427C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND PROBE FOR DETECTION OF HUMAN HERPES VIRUS 6B | 2022 |
|
RU2806428C1 |
| SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR THE DETECTION OF HUMAN HERPES VIRUS 6B | 2022 |
|
RU2802953C1 |
| METHOD FOR DETECTING AND LABORATORY CONFIRMATION OF INHERITED CHROMOSOMALLY-INTEGRABLE HUMAN HERPES VIRUS 6A/B | 2020 |
|
RU2739997C1 |
| OLIGONUCLEOTIDES FOR DETECTION OF SARS-CoV-2 MUTATION S:N501Y | 2022 |
|
RU2791958C1 |
| OLIGONUCLEOTIDES FOR DETECTION OF MUTATION S:delVYY143-145 OF SARS-CoV-2 | 2022 |
|
RU2795016C1 |
| OLIGONUCLEOTIDES FOR DETECTION OF SARS-CoV-70 MUTATION S:delHV69 | 2022 |
|
RU2795014C1 |
| METHOD OF GENOTYPING TLR2 GENE USING RS3804100 POLYMORPHISM AND A SET OF OLIGONUCLEOTIDE PRIMERS AND PROBES FOR ITS IMPLEMENTATION | 2023 |
|
RU2805860C1 |
