METHOD OF QUANTITATIVE DETERMINATION OF DNA OF HUMAN HERPES VIRUS 6A (HHV-6A) AND DNA OF HUMAN HERPES VIRUS 6B (HHV-6B) AND A SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR ITS IMPLEMENTATION Russian patent published in 2023 - IPC C12Q1/6806 G01N33/48 C12N15/38 

FIELD: medicine, molecular biology, biotechnology.

SUBSTANCE: group of inventions consists of a method of quantitative determination of DNA of human herpes virus 6A (HHV-6A) and DNA of human herpes virus 6B (HHV-6B), including extraction of DNA from a sample of biological material, real-time PCR reaction using a set of oligonucleotide primers and probes SEQ ID NO: 1–4 and DNA calibrators with a known concentration, amplification, analysis and interpretation of the results by constructing a calibration line and determining the concentration of target DNA in the test sample. The presented group also contains a set of oligonucleotide primers and probes for implementing the method. The kit has the following nucleotide composition: forward primer HHV-6A/B-for – SEQ ID NO: 1; reverse primer HHV-6A/B-rev – SEQ ID NO: 2; fluorescently labeled probe HHV-6А-probe – SEQ ID NO: 3; fluorescently labeled probe HHV-6B-probe – SEQ ID NO: 4.

EFFECT: presented group of inventions allows with 100% specificity to carry out species identification of human herpes viruses 6A (HHV-6A) and 6B (HHV-6B) with simultaneous quantitative assessment of the result.

10 cl, 3 tbl, 3 ex