METHOD OF QUANTITATIVE DETERMINATION OF DNA OF HUMAN HERPES VIRUS 6A (HHV-6A) AND DNA OF HUMAN HERPES VIRUS 6B (HHV-6B) AND A SET OF OLIGONUCLEOTIDE PRIMERS AND A PROBE FOR ITS IMPLEMENTATION Russian patent published in 2023 - IPC C12Q1/6806 C12N15/38 

FIELD: medicine; molecular biology; biotechnology.

SUBSTANCE: presented group of inventions is a method of quantitative determination of human herpes virus 6B DNA (HHV-6B) and a set. The method includes extraction of DNA from a sample of biological material, real-time PCR reaction using a set of oligonucleotide primers and probe SEQ ID NO: 1–3 and DNA calibrators with a known concentration, amplification, analysis and interpretation of the results by constructing a calibration line and determining the concentration of target DNA in the test sample. The set has the following nucleotide composition: a set of oligonucleotide primers and a probe for implementing the method of quantitative determination of human herpes virus 6B DNA (HHV-6B), having the following nucleotide composition: HHV-6A/B-for forward primer — SEQ ID NO: 1; reverse primer HHV-6A/B-rev – SEQ ID NO: 2; fluorescent probe HHV-6B-probe – SEQ ID NO: 3.

EFFECT: presented group of inventions allows with 100% specificity to carry out species identification of human herpes viruses 6A (HHV-6A) and 6B (HHV-6B) with simultaneous quantitative assessment of the result.

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