FIELD: molecular biology.
SUBSTANCE: method for parallel identification of 32 flax genetic markers associated with various economically useful traits is described by performing multiplex amplification of 32 flax genome regions carrying sites CP027619.1:4809251, CP027619.1:5296528, CP027619.1:10459658, CP027619.1:16726604, CP027619.1:18443786, CP027619.1:25260635, CP027621.1:17365579, CP027622.1:1035355, CP027623.1:15861094, CP027624.1:1082928, CP027624.1:15460619, CP027625.1:1935390, CP027625.1:1939072, CP027625.1:11374706, CP027626.1:5926678, CP027626.1:17310210, CP027626.1:22402660, CP027626.1:22756656, CP027627.1:3929632, CP027627.1:3929632, CP027627.1:6245953, CP027627.1:7262787, CP027627.1:19668773, CP027627.1:3929632, CP027627.1:24655347, CP027629.1:16437137, CP027630.1:5995854, CP027630.1:14198976, CP027630.1:13776301, CP027631.1:12047740, CP027631.1:16092048, CP027633.1:3857714 in the flax genome assembly GCA_000224295.2/ASM22429v2. To do this, primers represented by the sequences SEQ ID NO: 1 — SEQ ID NO: 64 are used, followed by analysis of amplification products using massively parallel sequencing technology and comparison of the obtained sequences with the reference alleles represented by the sequences SEQ ID NO: 65-96.
EFFECT: claimed invention makes it possible to simultaneously identify 32 loci in the flax genome associated with economically valuable traits.
1 cl, 1 dwg, 1 tbl, 3 ex
