FIELD: biotechnology.
SUBSTANCE: disclosed is a panel of oligonucleotides for producing a lyticase gene with a nucleotide sequence SEQ ID NO: 1, having the structure of oligonucleotide primers SEQ ID NO: 3–46, a lyticase gene having a nucleotide sequence coding the lyticase enzyme with SEQ ID NO: 1.
EFFECT: invention enables to obtain an expression construct coding lyticase and obtain lyticase in the form of a recombinant protein in Escherichia coli cells with high output of the enzyme in a soluble form.
5 cl, 2 tbl, 3 ex
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