FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, molecular genetics and molecular biology. Create a set of synthetic oligonucleotides, including nucleotide sequences: EcoAd1 5' TATTGACGCGCACAGCGAT 3', EcoAd2 5' AATTATCGCTGTGCGCGTCAATA 3', BisAd1 5' GGTCACGGATGCGCATT 3', BisAd2 5' NAATGCGCATCCGTGACC 3', KroAd1 5' CCGGGTCACGGATGCGCCTA 3', KroAd2 5' TAGGCGCATCCGTGAC 3', pBis 5' GTCACGGATGCGCCTANGC 3', pKro 5' CGCATCCGTGACCCGGC 3', pEco 5' ACGCGCACAGCGATAATTC 3' for specific labeling and amplification of methylated regions of DNA.
EFFECT: invention is used for synthesis, detection and subsequent sequencing of DNA with methylated CpG dinucleotides.
1 cl, 1 dwg, 1 ex
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