FIELD: medicine.
SUBSTANCE: method provides preparation of a protective medium of phosphate buffer of pH 7.2 containing glycerin, lactose, sodium citrate, gelatin. The prepared protective medium is mixed with lactic bacteria in the ratio 1:1 and kept for 15-20 minutes at temperature 17 to 27°C to be agitated, granulated and frozen in liquid nitrogen.
EFFECT: method improvement.
2 ex
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