FIELD: gynaecological oncology.
SUBSTANCE: mixture of recombinant E6-CBD and E7-CBD proteins incorporating the E6 and E7 antigens as well as the cellulose binding domain (CBD) from Caldicellulosiruptor danielii is described to provide a one-step isolation and purification technology. A simple and effective method has been developed for the production of recombinant proteins E6-CBD and E7-CBD, which includes binding to a cellulose-comprising sorbent due to affinity interaction, subsequent washing from unbound bacterial proteins and isolation of the target products. Based on a mixture of recombinant proteins E6-CBD and E7-CBD with the addition of various adjuvants, an immunogenic composition was obtained that induces the formation of a humoral and cellular immune response against HPV 16 and provides a pronounced antitumor effect in vivo. The use of an immunogenic composition for the treatment of malignant neoplasms associated with human papillomavirus type 16 (HPV 16) is disclosed.
EFFECT: expanding the range of drugs for the treatment of malignant neoplasms associated with human papillomavirus type 16.
4 cl, 3 dwg, 1 tbl, 4 ex
| Title | Year | Author | Number |
|---|---|---|---|
| MUCOSAL VACCINE FOR IMMUNE THERAPY OF DISEASES CAUSED BY HUMAN PAPILLOMA VIRUSES, AND RELATED THERAPY (VERSIONS) | 2008 |
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| RECOMBINANT PROTEIN COMPOSITION, METHOD FOR PREPARING SUCH COMPOSITION, PHARMACEUTICAL KIT OF REAGENTS FOR IMMUNOTHERAPY AND PROPHYLACTIC VACCINATION OF TUMOR DISEASES IN ANUS-GENITAL SPHERE, METHOD FOR IMMUNOTHERAPY AND PROPHYLACTIC VACCINATION BASED ON THEREOF | 2002 |
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| METHOD FOR EARLY AND PRECLINICAL DIAGNOSTICS OF CERVICAL CANCER | 2003 |
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| MEDICATION FOR TREATMENT OF UTERUS NECK DISEASES, ASSOCIATED WITH HUMAN PAPILLOMA VIRUS, IN FORM OF VAGINAL SUPPOSITORY | 2010 |
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| METHOD FOR ASSESSING CONDITIONS OF PROLIFERATIVE CERVICAL DISEASES | 2012 |
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| COMBINATION OF POXVIRUS ENCODING HUMAN PAPILLOMA VIRUS POLYPEPTIDES, AND IL-2 WITH ANTI-PD-L1 ANTIBODY | 2020 |
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| INTRACELLULAR DELIVERY OF BIOMOLECULES FOR IMMUNE RESPONSE MODULATION | 2019 |
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| METHOD OF PREDICTING PROGRESSION OF MILD INTRAEPITHELIAL NEOPLASIA IN WOMEN OF YOUNG AND FIRST PERIOD OF MATURE AGE | 2014 |
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| DRUG FOR HUMAN PAPILLOMA VIRUS ASSOCIATED DISEASES OF UTERINE NECK, PRESENTED IN FORM OF SOLUTION FOR VAGINAL IRRIGATIONS, KIT AND METHOD FOR PREPARING | 2010 |
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