FIELD: molecular biology.
SUBSTANCE: method for detecting Helicobacter pylori infection and its resistance to clarithromycin and levofloxacin in a biopsy sample of the gastric mucosa of a patient is described. The method includes carrying out a polymerase chain reaction using a PCR reaction solution and primers, purifying the PCR product, sequencing reaction, purifying the sequencing reaction product, detecting and analyzing the result, while the test system contains a set of reagents for PCR, buffer, MgCl2, dNTP, Taq polymerase, DNA sample and primer set.
EFFECT: increasing the accuracy of diagnosing Helicobacter pylori infection and its resistance to clarithromycin and levofloxacin.
1 cl, 6 dwg, 3 ex
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