ANALYSIS METHOD OF EML4-ALK TRANSLOCATIONS ASSOCIATED WITH SENSITIVITY OF LUNG CANCER TO ANTITUMOUR TARGET THERAPY Russian patent published in 2014 - IPC C12N15/00 C12Q1/68 

FIELD: biotechnologies.

SUBSTANCE: proposed method involves the following: obtaining EML4-ALK cDNA by means of a polymerase chain reaction with reverse transcription (RT-PCR) on RNA matrix of EML4-ALK gene using specific primers; amplification of fragments of EML4-ALK gene by means of a multiplex PCR method on cDNA matrix obtained at the first stage of RT-PCR by means of high-specific primers; obtaining fluorescent-marked PCR-product at the second stage of RT-PCR; creation of a biochip for analysis of EML4-ALK translocations, which contains a set of immobilised probes; hybridisation of fluorescent-marked PCR-product with probes in gel cells on a plastic substrate of the biochip; recording and interpretation of hybridisation results. The method provides for use of a technology of DNA biochips designed for the purpose of determining 6 versions of EML4-ALK translocations (V2, V3, V5, V4, V7, V1).

EFFECT: method has high sensitivity and specificity; it is general-purpose, and its implementation is possible with operating material and tumour tissue enclosed in paraffin units; recording of results is performed on a single occasion at the end of the investigation; the method's implementation lasts for less than 24 hours.

3 dwg, 3 tbl, 3 cl