FIELD: biotechnology.
SUBSTANCE: invention relates to biotechnology, the method includes combined reverse transcription of RNA samples isolated from formaldehyde-fixed tissue in paraffin blocks, and amplification of their cDNA in real time using the developed set of oligonucleotide primers and fluorescently labeled probes.
EFFECT: invention makes it possible to carry out differential diagnosis of Ewing's sarcoma, reduces the time spent on the analysis, and reduces its cost.
2 cl, 2 dwg, 2 tbl, 2 ex
| Title | Year | Author | Number |
|---|---|---|---|
| L-AMINO ACID PRODUCING MICROORGANISM AND METHOD FOR PRODUCING L-AMINO ACID WITH ITS USE | 2020 |
|
RU2795161C1 |
| METHOD OF AMPLIFICATION AND IDENTIFICATION OF NUCLEIC ACIDS | 2019 |
|
RU2811465C2 |
| NEW VARIANT OF GLUTAMINE HYDROLYZING GMP SYNTHASE AND A METHOD OF PRODUCING PURINE NUCLEOTIDE USING IT | 2021 |
|
RU2804010C1 |
| TANDEM PROMOTER FUNCTIONING IN BACTERIA OF BACILLUS GENUS, TRANSFORMED BACILLUS BACTERIUM - TARGET PRODUCT PRODUCER CONTAINING SAID PROMOTER, AND METHOD FOR PRODUCING DESIRED PRODUCT USING SAID BACTERIUM | 2019 |
|
RU2723722C1 |
| PHARMACEUTICAL COMPOSITIONS, KITS AND METHODS OF TREATING TUMORS | 2019 |
|
RU2810906C2 |
| MICROORGANISMS FOR PRODUCING L-TYROSINE AND METHOD FOR PRODUCING L-TYROSINE WITH THE USE THEREOF | 2020 |
|
RU2779461C1 |
| METHOD FOR PRODUCING AN AGENT OF THE RIBONUCLEOPROTEIN COMPLEX CRISPR-Cas12 AND AGENT FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS IN ULTRA-LOW CONCENTRATIONS | 2021 |
|
RU2792891C1 |
| NEW PROMOTER AND ITS USE | 2021 |
|
RU2811953C1 |
| ANTISENSE OLIGONUCLEOTIDES TO ALPHA-SYNUCLEIN AND APPLICATIONS THEREOF | 2019 |
|
RU2773197C2 |
| METHOD FOR DETECTING THE DNA OF THE HEPATITIS B VIRUS AT ULTRA-LOW CONCENTRATIONS AND SPECIFIC OLIGONUCLEOTIDES FOR USE IN THE METHOD | 2021 |
|
RU2782951C1 |
