FIELD: optical instrumentation.
SUBSTANCE: optical system for detecting nucleic acid amplification products. The optical system contains an optical block housing in which optical spectral channels are located consisting of transmitting and receiving optical paths. The transmitting optical path consists of at least one light source, a light filter, as well as a dichroic mirror or beam splitter, which ensures alignment of the optical axes of the receiving and the transmitting optical paths. The receiving optical path consists of a dichroic mirror or a beam splitter common with the transmitting path, a light filter, and a detector. The optical unit is configured for reciprocating rotational movement along a sector of a circle so that the radius of the trajectory of this movement coincides with the radius at which the centres of the reaction wells of the microfluidic chip are located. This ensures positioning of each optical spectral channel above each of the reaction wells.
EFFECT: simpler design and improved reliability, as well as ability to simultaneously register signals from various fluorescent dyes.
13 cl, 3 dwg
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Authors
Dates
2023-12-12—Published
2023-03-30—Filed