FIELD: agriculture.
SUBSTANCE: crop production and experimental biology. In the method, the leaf and standards are illuminated with a directed light flux from the source, and the intensity of the reflected mixed flux and its diffuse component are measured. The leaf is placed on a cylindrical surface so that the central vein of the leaf is located along the axis of the cylinder. A digital camera located opposite the center of the leaf is used as a receiver of reflected light. Two digital images are obtained with symmetrical positions of the light source at an angle of 20 degrees on one side and the other from the camera’s line of sight to the center of the leaf. The images are calibrated using images of the reference surfaces in the images. Based on the known values of their reflection coefficients, the reflection coefficients of sections of the leaf surface are determined. The reflection coefficients of sections of the leaf surface are measured by the brightness of the corresponding pixels in the images along lines perpendicular to the axis of the cylindrical surface. Areas of the image of the leaf surface on lines parallel to the cylinder axis are used to obtain repeat measurements; the area of mixed reflection is identified on the indicatrix of reflection coefficients based on the maximum value of the reflection coefficient. A section of the leaf surface located symmetrically to the selected area of mixed reflection relative to the main vein of the leaf is taken as a diffuse reflection region. As a bilateral feature, the ratio of the mixed and diffuse reflection coefficients of the selected symmetrical areas is used. The functional state of the plant is judged by the average value of the found bilateral trait, expressed in relative units, with differences in coefficients of less than 5% - an insignificant effect on the functional state of the plant. The stability of plant development is judged by the fluctuating asymmetry of a bilateral trait, an increase of which by 30% indicates the influence of the spectrum on the stability of plant development.
EFFECT: implementation of environmental monitoring of plant stress conditions, expanding functionality, increasing the efficiency, reliability and accuracy of measurements, and reducing their labor intensity.
1 cl, 6 dwg, 2 ex
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Authors
Dates
2023-12-27—Published
2023-05-05—Filed