FIELD: biotechnology.
SUBSTANCE: invention relates to a method of obtaining of a transgenic mouse cell containing an artificial recombinant chromosome, which involves preparing a human cell containing the first human chromosome, processing the human cell to obtain a constructed human cell, processing a constructed human cell to obtain the first human microcell containing a first engineered human chromosome, preparing a mouse ES cell containing the first mouse chromosome, treating the mouse ES cell to produce an engineered mouse ES cell containing a second engineered mouse chromosome, contacting the engineered mouse ES cell with the first human microcell, and use of site-specific recombinase (SSR). Also the following is disclosed: a method of obtaining a transgenic mouse using a transgenic mouse cell including an artificial recombinant chromosome.
EFFECT: invention is effective for obtaining a mouse cell containing an artificial recombinant chromosome.
10 cl, 35 dwg, 5 tbl, 3 ex
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