METHOD FOR SELECTING INDIVIDUALS WORKING IN CONDITIONS OF EXPOSURE TO SILICON-CONTAINING AEROSOLS FOR SUBSEQUENT MONITORING OF STATE OF BRONCHOPULMONARY SYSTEM Russian patent published in 2024 - IPC G01N33/68 G01N33/52 G01N1/28 G01N21/77 

FIELD: medicine.

SUBSTANCE: invention relates to medicine, namely to occupational pathology, pulmonology, occupational hygiene, and can be used for selecting individuals working in conditions of exposure to silicon-containing aerosols for subsequent monitoring of the state of the bronchopulmonary system. Medical examination is carried out by determining the diagnostic laboratory and functional values. To obtain diagnostic laboratory values, blood is sampled in the morning on an empty stomach by venipuncture of an ulnar vein into a vacuum test tube with heparin, immediately after filling the test tube with blood is placed on ice. Before analysis, the sample is thoroughly mixed by turning test tube 8–10 times. In a clean plastic test tube 1.0 ml of blood is collected, 1.5 ml of cold deionised water is added, thoroughly mixed and maintained for 15 minutes at 4 °C. 0.650 ml of 25% sulphosalicylic acid solution is added to the test tube, thoroughly mixed and centrifuged at 4 °C for 10 minutes at 10,000 rpm. Obtained supernatant is used to determine the amount of reduced and total — after reduction of oxidised glutathione by a spectrophotometric method using Ellman's reagent. Measurement is carried out on a CLIMA MC-15 semi-automatic biochemical analyser. Standards-calibrators are used to calculate concentration of reduced and total glutathione in whole blood. Concentration of oxidised glutathione is calculated by subtracting concentration of reduced glutathione from concentration of total glutathione, the obtained result is divided by two. Ratio of the concentration of reduced glutathione to the concentration of oxidised glutathione is calculated; result is expressed in units. Individuals exposed to silicon-containing aerosols with a coefficient less than 10.0 units are to be included in the clinical examination group for subsequent monitoring of the state of the bronchopulmonary system.

EFFECT: method enables easy implementation and high efficiency of the procedure for selecting persons working in contact with industrial aerosols, during mandatory medical examinations for subsequent monitoring of the state of the bronchopulmonary system by determining the concentration of reduced and total glutathione in the blood tested with subsequent calculation of concentration of oxidised glutathione and ratio of concentration of reduced to concentration of oxidised glutathione.

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