METHOD OF DECELLULARIZATION OF PERIPHERAL NERVE FOR RECONSTRUCTIVE SURGERY IN EXPERIMENT Russian patent published in 2024 - IPC A61L27/14 A61L27/22 A61L27/38 

FIELD: medicine.

SUBSTANCE: invention relates to a method of decellularization of a peripheral nerve. A method of decellularizing a peripheral nerve for reconstructive surgery in an experiment, including nerve sampling, characterized by the following: the sciatic nerve is taken from a rat and frozen at a temperature of -70–80°C followed by defrosting; treated with Trypsin-Versene solution at a temperature of +37°C on a shaker-incubator at 100–150 rpm for 6 hours, with periodic changes of solutions every 2 hours; successively double exposure on a shaker at 100–150 rpm with solutions of 1% Triton X-100 for 3 hours and 4% sodium deoxycholate for 3 hours, with the condition of washing the samples with distilled water after cycles for 5–20 minutes and subsequent processing pork pancreatic DNase for 4 hours at a temperature of +37°C on a shaker-incubator at 100–150 rpm, while the nerve is treated in the indicated solutions in the ratio of the sample mass in grams to the volume of solutions in milliliters — 1:10 for 22–23 hours, then the quality of discoloration is monitored and provided DNA content of no more than 50 ng/mg of tissue, the samples meet the specified requirements, and if the sample does not meet the specified requirement, it is processed a second time in the same modes with solutions of Triton X-100 and sodium deoxycholate.

EFFECT: ensuring the production of material for replacing peripheral nerve defects with the maximum preserved histological structure of the native nerve, which does not have immunogenic properties, which does not injure the donor site, prevents the formation of painful neuroma, allows nerve impulses to be transmitted from one end of the nerve to the other, without allowing the distal part to die process.

1 cl, 2 ex