METHOD FOR PREDICTING THE DEVELOPMENT OF IRON DEFICIENCY ANEMIA (VARIANTS) Russian patent published in 2002 - IPC

FIELD: medicine, hematology. SUBSTANCE: it is conducted the analysis of polymorphous loci of P-4501A1 cytochromic gene due to polymerase chain reaction of DNA synthesis, it is conducted in DNA isolated due to phenol-chloroform extraction technique out of lymphocytes of peripheral venous blood. After amplification and extraction DNA fragments are electrophoretically divided in 7%- polyacrylamide nondenaturated gel and analyzed under ultraviolet lighting by using a transulluminator: the presence of DNA-fragments at the size of 48 and 139 nucleotide pairs (np) is interpreted as isoleucin allele (Ile), at the size of 48, 120 and 19 np - as valine (Val). Relative risk of disease development (RR) is concluded on at RR>1.5. At revealing Ile/Val genotype it is predicted the risk of IDA development (variant 1). Then it is conducted the analysis of polymorphous loci of glutathione S-transferase M1 genes (GSTM1), N-acetyltransferase 2 (Nat2), angiotensin converting enzyme (ACE) and plasminogene activator (PA) due to the method of polymerase chain reaction of DNA synthesis (PCR) and at revealing genotype combinations of 0/0,S/R,I/D,I/D; +/+,R/R,I/I,I/D; +/+,S/S,I/D,I/D; +/+,S/R,I/I, I/D; +/+,S/R,D/D,I/I the risk of iron deficiency anemia development is predicted. EFFECT: higher accuracy of prediction. 2 cl, 2 ex, 1 tbl